cromolyn sodium (cs)  (Entod Pharma Ltd)

Journal: JOR Spine

Article Title: Characterization and modulation of the pro‐inflammatory effects of immune cells in the canine intervertebral disk

doi: 10.1002/jsp2.1333

Figure Lengend Snippet: Culture groups.

Article Snippet: In addition to no treatment controls, canine MCs were pre‐treated with 25 μM of cromolyn sodium (CS; Cayman Chemical 21 379), a mast cell stabilizer, 48 h prior to seeding with canine NP cells in 3D agarose gel (NP + MC + CS) as described above.

Techniques:

Journal: JOR Spine

Article Title: Characterization and modulation of the pro‐inflammatory effects of immune cells in the canine intervertebral disk

doi: 10.1002/jsp2.1333

Figure Lengend Snippet: (A) Effect of stiffness (1% and 4%) on gene expression of nucleus pulposus (NP) cells at 14 days and 21 days ( N = 3–6). (B) Effect of stiffness (1% and 4%) on gene expression of mast cells (MCs) at 14 days and 21 days ( N = 3–6). (C) Effect of stiffness (1% and 4%) on gene expression in the NP + MC co‐culture at 14 and 21 days ( N = 3–6). (D) Effect of stiffness (1% and 4%) on gene expression in treatment groups NP + MC + protease activated receptor 2 antagonist (PAR2A) and NP + MC + cromolyn sodium (CS) + P at 21 days ( N = 3–6). * p value <0.05, ** p value <0.005. All error bars indicate standard error mean. Statistics = linear mixed model with Benjamini–Hochberg.

Article Snippet: In addition to no treatment controls, canine MCs were pre‐treated with 25 μM of cromolyn sodium (CS; Cayman Chemical 21 379), a mast cell stabilizer, 48 h prior to seeding with canine NP cells in 3D agarose gel (NP + MC + CS) as described above.

Techniques: Gene Expression, Co-Culture Assay

Journal: JOR Spine

Article Title: Characterization and modulation of the pro‐inflammatory effects of immune cells in the canine intervertebral disk

doi: 10.1002/jsp2.1333

Figure Lengend Snippet: Effect of treatment (cromolyn sodium [CS] and/or protease activated receptor 2 antagonist [PAR2A]) on gene expression in the 4% stiffness agarose gel at 14 and 21 days ( N = 3–6). * p value <0.05. All error bars indicate standard error mean. Statistics = linear mixed model with Benjamini–Hochberg. MC, mast cell; NP, nucleus pulposus.

Article Snippet: In addition to no treatment controls, canine MCs were pre‐treated with 25 μM of cromolyn sodium (CS; Cayman Chemical 21 379), a mast cell stabilizer, 48 h prior to seeding with canine NP cells in 3D agarose gel (NP + MC + CS) as described above.

Techniques: Gene Expression, Agarose Gel Electrophoresis